3D printing of vascularized hepatic tissues with a high cell density and heterogeneous microenvironment
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Published:2023-07-20
Issue:4
Volume:15
Page:045004
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ISSN:1758-5082
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Container-title:Biofabrication
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language:
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Short-container-title:Biofabrication
Author:
Fang Yongcong,
Ji Mengke,
Yang Yi,
Guo Yihan,
Sun Ruobin,
Zhang Ting,
Sun Wei,
Xiong ZhuoORCID
Abstract
Abstract
Three-dimensional bioprinting has emerged as an appealing approach for creating functional tissues; however, a lack of suitable bioinks with high cell density and printability has greatly limited our ability to print functional tissues. We address this limitation by developing a granular cell aggregate-based biphasic (GCAB) bioink based on densely packed cell aggregates. The GCAB bioink exhibited the desired shear-thinning and shear-recovery properties for extrusion bioprinting and hyperelastic behaviors postprinting for modeling the mechanical characteristics of soft biological tissues. The GCAB bioink displayed a high cell density (∼1.7 × 108 cells cm−3) without compromising viability (∼83%). We printed dense hepatic tissue constructs with enhanced vascularization and metabolic functions by preorganization of GCAB bioink with a defined heterogeneous microenvironment. By simultaneously printing the GCAB bioink and an endothelial cell-laden gelatin bioink, we successfully produced functional hepatic tissues with a high cell density and a perfusable vascular network. The design of the generalizable GCAB bioink opens new avenues to create functional tissues for therapeutic applications.
Funder
New Faculty Start-up Funding of Tsinghua University
National Natural Science Foundation of China
China Postdoctoral Science Foundation
National Key Research and Development Program of China
Subject
Biomedical Engineering,General Medicine,Biomaterials,Biochemistry,Bioengineering,Biotechnology
Cited by
1 articles.
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