Abstract
Abstract
The micro gas chromatography columns (μGCs) were
prepared for rapid breath analysis of gastric cancer. The
synergistic effect of the specific surface area and the action of
pore diameter on the separation capacity was investigated. The
μGC-IL/UIO-66 was prepared using [P66614][Cl]/UIO-66 as the
stationary phase. For comparison, the μGC-IL and the
μGC-UIO-66 were prepared using [P66614][Cl] and UIO-66 as
stationary phase, respectively. [P66614][Cl]/UIO-66 had a high
specific surface area with a pore diameter distribution of
0.49 nm. The high specific surface area of [P66614][Cl]/UIO-66
improved the efficiency of adsorption and desorption, while the
porous structure with an appropriate pore diameter acted as an
efficient molecular sieve, synergistically enhancing separation
efficiency. So compared to the μGC-IL and the
μGC-UIO-66, the HETP of μGC-IL/UIO-66 was reduced by
68.2% and 22.6%, respectively. In the analysis of volatile
biomarkers (acetone, benzene, n-hexane and toluene) for gastric
cancer, the resolutions between adjacent peaks were 1.96, 2.13 and
3.67, which met the requirements for quantitative analysis
(R > 1.5). The retention times of acetone, benzene, n-hexane and
toluene were 0.72 min, 0.96 min, 1.33 min and 1.67 min, which
enables rapid analysis. All may suggest that the μGC-IL/UIO-66
has a promising application in rapid breath analysis of gastric
cancer.