Synthesis, capping and binding of colloidal gold nanoparticles to proteins

Author:

Nghiem Thi Ha Lien,La Thi Huyen,Vu Xuan Hoa,Chu Viet Ha,Nguyen Thanh Hai,Le Quang Huan,Fort Emmanuel,Do Quang Hoa,Tran Hong Nhung

Abstract

Abstract Bovine serum albumin (BSA) was used as a stabilizing agent and biofunctionalized layer for water-dispersed gold nanoparticles (NPs) synthesized from metal precursor HAuCl 4. The BSA binding to gold NPs was characterized qualitatively and quantitatively by transmission electron microscopy, UV-VIS and FTIR spectrophotometers. HER2 (human epidermal growth factor receptor 2) specific phage antibodies were attached to BSA stabilized gold NPs to form a gold–antibody complex. An ELISA (enzyme-linked immunosorbent assay) test was done to confirm the bioactivity of antibodies attached to gold NPs.

Publisher

IOP Publishing

Subject

Electrical and Electronic Engineering,Industrial and Manufacturing Engineering,General Materials Science

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