Comparison of two rapid DNA extraction for DNA based identification from fish fillet products

Abstract

Abstract Efficiency and quality on the result of DNA extraction determined the success of species identification using DNA. High concentration and good purity of DNA are the basic principle for choosing the appropriate extraction method. The objective of this research is to find an effective and efficient method from two different rapid DNA extractions, KAPA Express Extract Kit (KAPA Biosystem) and Chelex® 100 (Biorad). We used each method to extract 44 fish fillet samples from Jabotabek (Jakarta, Bogor, Tangerang, Bekasi) modern retail and traditional markets. We found the KAPA Express Extract Kit (KAPA Biosystem) had fast extraction time approximately 15 minutes for PCR-ready DNA faster than 20 minutes using the Chelex® 100 (Biorad) method. All the PCR-ready DNA are visualized with gel electrophoresis and both method resulted in positive DNA bands. We propose the use of KAPA Express Extract Kit (KAPA Biosystem) than the Chelex® 100 (Biorad) method because the fast and easily to use for the next analyses such as PCR and DNA sequencing.