Optimum response of melinjo peel (gnetum gnemon) antioxidant activity using response surface methodology (RSM)

Abstract

Abstract Melinjo (Gnetum gnemon) is one of plants provided in abundant numbers. Melinjo peel contained antioxidant compound which is beneficial for the human body health. This compound protects the body cells from free radicals. This research aims to optimize the antioxidant activity in the Melinjo peel tea through Response Surface Methodology with Central Composite Design (CCD). The design consisted of 13 combination treatments with variable of 0.05% - 0.1% citric acid concentration levels and 5-10 minutes of blanching duration levels. The result of antioxidant activity of the treatment design was 75.21-87.6%. The antioxidant activity of melinjo peel tea was analyzed through DPPH method with 518 nm of spectrophotometer wave length. The optimum point was obtained through 0.075% citric acid concentration and 7.50 minutes of blanching duration with 78.68% antioxidant activity while the verification result of antioxidant activity was 76.37%. This had fulfilled the fit requirement by not exceeding the 5% margin of error.