In Vitro Regeneration of Stevia Rebaudiana Bertoni from internode and leaf explants using different concentrations of BAP (6-Benzyl Amino Purine)

Abstract

Abstract The aim of this study was to determine the response of the growth of internode explants and leaves of Stevia (Stevia Rebaudiana Bertoni), which were cultured at several BAP concentrations. This study was designed using a non Factorial Complete Randomized Design that is by testing the internode and leaf explants at 3 different BAP concentration levels of 2, 3 and 4 ppm and will be repeated 10 times. The parameters observed related to callus growth include: emergence, type, color and percentage of callus formation. In addition, observations were also made on the parameters of bud growth including the appearance of shoots, number and length of shoots. The results of this study indicate that 100% of leaf and internode explants are able to form callus. The color of the callus explants on average leaves are yellowish green while the internode explants are yellowish white and all have compact textures. In shoot growth parameters, only internode explants were able to grow shoots while leaf explants were not. The emergence of shoots (3 DAP), number of shoots (8.70), and shoot length (2.81cm) were produced by MS media with the addition of 3ppm BAP.