A study of the Relationship of Polymorphisms of the PPARGC1A Gene with Milk Production and its Components, in Holstein Friesian Cows

Abstract

Abstract This research was done at a Molecular Genetics lab at the College of Agriculture at the University of Basrah. The objective of this study is to determine how Holstein Friesian cows’ PPARGC1A genetic polymorphism affects milk production and its constituent parts. For milk production and quality standards, 59 Holstein Friesian cows from Taj Al-Nahrain station in Al-Diwaniyah are employed. Blood was drawn for genomic DNA, and the PPARGC1A gene was amplified using PCR. The samples were then transferred to the Korean firm Macrogene for DNA sequencing analysis. Morning milk 50ml samples were collected once each week., and after taking the samples during the milking process, they were evaluated for quality using the Julie Z7 milk analyzer. Association analysis was performed using SPSS statistical software. According to the current study’s findings, the stage of milk production had a significant impact on both daily and total production (p ≤0.05). The results also shows that there is a significant differences (p≤0.05) of stage of milk production on the chemical composition of milk, while there are no significant differences between Genetic Polymorphisms in daily and total milk production. The results show that hap1 is superior on hap2 in the chemical contents of milk, except for the percentage of milk fat, which has not reach a significant level.