Author:
Essa A. H.,Mohammed T. R.,Al-Anbari N. N.
Abstract
Abstract
Bone protein receptor gene (BMPR1B) is a significant fertility gene found in sheep globally and associated to reproductive and fertility features. This study verified the relationship between the BMPR1B gene’s 893 G A SNP1, SNP2, SNP3, and SNP4 C T 893. 35 Awassi ewe blood samples were acquired from the Agricultural Research Department in Anbar Governorate – Al Dawar, followed by DNA extraction to validate the BMPR1B gene and PCR amplification. The Sanger Sequences technique of single nucleotide polymorphism (SNP) was employed to determine each ewe’s special alleles using the gene primer. Four BMPR1B gene sites had substantially varied genotype and allele frequencies (P < 0.01). The association study indicated that the rs429976637 (G>A) locus had three polymorphisms, GG, GA, and AA, with percentages of 92.16, 7.68, and 0.16%, respectively, and allelic frequencies of G - 0.96 and A-0.04. Polymorphisms differed (P=0.01). rs416717560 (G>A) had three polymorphisms: GG, GA, and AA. The allelic frequency of the G alleles was 0.91, and polymorphism was 82.82, 16.38, and 0.80%. Polymorphism differed by 0.09 (P=0.01). The rs403555643 locus (A>G) has three polymorphisms, AA, AG, and GG, with percentages of 92.14, 7.68, and 0.16%, respectively, and allelic frequencies of A-0.96 and G - 0.04 (P < 0.01). The locus rs427046713 (C>T) had three polymorphisms, CC, CT, and TT, with percentages of 94.09, 5.82, and 0.09%, respectively. The allelic frequency of C alleles was 0.97- and T 0.03--P < 0.01. The allelic frequency of the BMPR1B gene for four loci is considerably greater for genotypes GG, GG, AA, and CC.
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