Impact of Different Levels of Insulin on Cryopreservation Local Roosters Stored Semen Biomarkers

Abstract

Abstract The aim of this research to examine the impact of different level of Insulin certain biomarkers of local roosters semen after being cooling for varying lengths of periods, roosters semen pooled, diluted with extender, and distributed randomly among five groups: Diluted semen put through the semen cryopreservation technique; the control treatment (C1) 0 insulin; (C2) contained 4 IU/insulin; (C3) contained 5 IU/insulin; (C4) contained 6 IU/insulin; and (C5) contained 7 IU/insulin. After 0, 24, 48 and 72 hours, cooling semen were evaluated for plasma membrane integrity(MI), acrosome integrity(AI), malondialdehyde (MDA) and total antioxidant capacity (TCA). The result showed significant differences between the treatments in terms of MI, AI and TCA in sperm with C5, C3, C2 respectively, but there were no significant differences in terms of MDA. TCA were found to be significantly improved in the fourth storage period.