Gene Expression of HSP70 Gene when Ascorbic Acid is Added to Broiler Diets Meat and Its Relationship to Meat Quality

Abstract

Abstract This experiment was conducted in the animal production field of the Agricultural Research Station/College of Agriculture and Marshlands/Dhi Qar University for the period from (30/11/2022 to 4/1/2023) for the purpose of studying the gene expression of the HSP 70 heat shock gene when ascorbic acid was added to the diets provided to birds. And the extent of its effect on the quality of meat. In this study, 150 non-sexed Ross 308 birds, one day old, were used in this study. The study included four treatments. Incubation was randomly distributed among the experimental treatments, with three replicates of 12 replicated birds. The treatments were divided on the basis of adding ascorbic acid to the diet without adding it, as well as shedding heat by 2-3 m for a period of 8 hours / day at the normal temperature rate and without heat shedding. The division of treatments began immediately after the second week of the age of the birds. The gene expression level of the HSP70 gene was measured at the age of 21 and 35days, and the sensory evaluation test of the thigh and chest segments was measured at the age of 21. and 35 days The results of the study showed the: 1-The high rate of gene expression of the HSP70 gene in the liver tissue in the T2 treatment exposed to a high temperature negatively affected the quality of meat through conducting a sensory evaluation of the thigh and breast segments, where the evaluation rate decreased in this treatment, which indicates that the relationship is inverse between the rate of the gene HSP70 and average sensory evaluation of the thigh and thoracicseg ments. 2-Adding ascorbic acid at a rate of 1 gm / kg feed to the T4 treatment that was also exposed to high temperature erecorded an increase in the sensory evaluation rate of the thigh and breast segments, which led to an improvement in flavor, taste and tenderness in poultry meat, which indicates that the addition of ascorbic acid has a role in relieving stress Thus decreased, the rate of gene expression of the HSP70 gene.