In vitro anthelmintic activities of shrub plants extracts for Haemonchus contortus worms

Abstract

Abstract This study was conducted to evaluate the anthelmintic activity and effectiveness of shrubs as forage containing tannins as anti-parasites in the digestive tract of small ruminants by in vitro. The types of shrubs used in the study included anting-anting (Acalypha australis linn), bandotan (Ageratum conyzoides), som jawa (Talinum paniculatum), suruhan (Peperomia pellucid L.), and tapak liman (Elephantopus scaber). This study at P0: aquadest (negative control) P1: 10% concentration level of shrubs; P2: 20% concentration level of shrubs, and P3: albendazole 100% (positive control) after 60 minutes of time. The methods of Haemonchus contortus adult worm mortality test were used to investigate the inhibiting effect of in vitro worm mortality from shrub plant extracts. The results of this study on in vitro screening of adult worms, at an increasing concentration, the number of worms that died was significantly more (P <0.05). The highest worm mortality was seen at the concentration level of tapak liman leaf extract (Elephantopus scaber) as much as 20% with a tannin content of 8.89% so that the anthelmintic potential was seen in adult worm mortality. From this study, it can be concluded that the extract of shrubs shows significant anthelmintic activity against the death of adult female Haemonchus contortus worms so that it has the potential to play a role in parasite control in the digestive tract of small ruminants.