Enrichment of anaerobic and facultative anaerobic bacteria from a graphite mine sample on graphene oxide

Abstract

Abstract Graphene oxide (GO) is progressively synthesized and applied in various industrial fields, such as healthcare, medicine, gas transport, electric power industry, etc. Thus, its distribution in the environment increases, which leads to changes in various microbial communities. Mechanisms of interaction between bacterial communities and novel carbon-based nanomaterials, such as GO, are still to be elucidated. We developed stable consortia in order to identify bacteria with the ability to thrive in the presence of GO. Here, we show the results of metagenomic analyses of the graphite mine sample, a consortium with GO as a sole source of carbon and a consortium with fumarate as an additional carbon source. The aim of this study was to establish anaerobic enrichment cultures from a graphite mine sample and GO, and to identify their diversity/community composition.