Citrus x microcarpa bunge fruit extract as antibacterial against staphylococcus aureus

Abstract

Abstract Citrus x microcarpa Bunge is a plant that is used optimally by the people of Bangka Belitung known as the ‘Jeruk Kunci’. Utilization of this plant in the fruit is used as an acid enhancer in food and is made in the form of syrup. So that the jeruk kunci fruit waste is widely available and has not been utilized.based on literature, Citrus x microcarpa contains flavonoids and a monoterpeneshydrocarbon. Bioactivity which belongs asantimicrobial, antibacterial and antioxidant. Therefore, jeruk kunci fruit waste has the potential to be developed as an antibacterial herbal medicine based on literature studies. Citrus x microcarpa Bunge extract was obtained by maceration for 3 x 24 hours using ethanol solvent. Then phytochemical testing was conducted qualitatively using several reagents and quantitative phytochemical testing was carried out using functional group analysis contained in the extract uses the Fourier Transform Infrared (FT-IR) at the wavenumber region 4000-400 cm-1. Then, determination of antibacterial activity using the disk diffusion method. Based on FT-IR spectrum data analysis Citrus x microcarpa extract contains tannins. The results of antibacterial testing of Citrus x microcarpa Bunge extract obtained concentration of 20%, 40% and 60% has a relatively strong antibacterial inhibition. whereas for 80% and 100% concentrations the ability to inhibit is very strong.