Evaluation of Inoculation with Bradyrhizobium manausense and Bradyrhizobium venae on the Elemental content of Cowpea Plant in Gypsum Soil

Abstract

Abstract A field experiment was conducted during the spring season on 3/25/2022 in the fields of the University of Tikrit / College of Agriculture located in the research station of the Department of Soil Sciences and Water Resources, with the aim of studying the effect of inoculation with Rhizobia bacteria and nitrogen fertilization on the growth and yield of cowpea in gypsum soil, conducted in a randomized complete block design. R. C. B. D With three replications, the experiment included two main factors: inoculation with rhizobium bacteria, and the second factor was nitrogen fertilization. The inoculation factor was three levels (A0 = no inoculation, A1 = inoculation with the first strain, Bradyrhizobium manausense, and A2 = inoculation with the second strain, Bradyrhizobium vignae), and the second factor was nitrogen fertilization. At three levels N0 and N1 = 40 kg / ha and N2 = 80 kg / ha, the process of isolation and development of Rhizobium bacteria was conducted on the optional medium (YEMA), and the two isolates were identified and recorded in the National Center for Biotechnology Information (NCBI) by number Global OP740389 Bradyrhizobium vignae, as for the results of the field experiment, the inoculation treatment with root nodes bacteria Bradyrhizobium vignae was superior to the control treatment and even to the second strain Bradyrhizobium manausense in all studied traits of cowpea plant, and the percentage increase of the inoculation treatment with Bradyrhizobium vignae with 40 kg of nitrogen fertilizer was higher than The control treatment in the number of root nodes and their wet weight (1474.87, 267.101)%, respectively, and the concentration of nitrogen, phosphorus and potassium in the vegetative part (61.300, 1.600, 7.102)%, respectively.