Morphological, Biochemical and Molecular Identification Bacteria Streptomyces spp. Causing Common Scab Disease on Potato in Iraq

Abstract

Abstract This study was carried out at the University of Anbar - College of Agriculture - Department of Plant Protection. The study aimed to isolate and diagnose the bacterium that causes scabies on potatoes, Streptomyces spp., depending on the phenotypic characteristics, biochemical interactions, and molecular methods. The results of isolation from different regions and on PDA medium showed that it was obtained On 45 pure isolates of the pathogenic bacteria Streptomyces spp, and the results of growth in different media showed that the best medium for growth was PDA and Nutrient agar. The results of phenotypic diagnosis and biochemical tests revealed that 8 isolates belong to the type Streptomyces scabies and two isolates belong to the type Streptomyces acidiscabies. DNA was extracted from 10 isolates of pathogenic bacteria, and the results of molecular diagnosis using the 16S rRNA gene showed that light bands of 1250 pairs were obtained. The nitrogenous base sequences of these isolates were determined and deposited in GenBank under accession codes (OQ8191, OQ819106, OQ815918, OQ789975, OQ789966, OQ815949, OQ815950).