Application of coumarin concentrations and number of nodes on induction potato (Solanum tuberosum L.) micro tuber in vitro

Abstract

Abstract Potato is a horticultural commodity with good prospects for development. Potato tissue culture technology is one of the efforts to produce virus- and disease-free potato seeds. The aim of this experiment was to see if there was an interaction between the administration of multiple coumarin concentrations and the number of nodes and at what concentration of coumarin and at what number of nodes gives optimal results for induction of potato micro tuber. This experiment was arranged according to a two-factor factorial Completely Randomized Design with 3 replications. The 1st factor was multiple coumarin concentrations with 4 levels (30, 60, 90, 120 mg/l) and the 2nd factor was the number of nodes with 3 levels (1 node, 2 nodes, and 3 nodes). The results showed that there was an interaction between the administration of multiple concentrations of coumarin and the number of nodes, showing the best interaction when the coumarin concentration was 120 mg/l with 2 nodes that could form tubers with a percentage of 94.44%. The 120 mg/l coumarin concentration gave the highest of plantlets forming micro-tubers, the highest number of micro tuber and the largest micro-tuber diameter. The two-node treatment increased the percentage of plantlets forming micro tubers, the number of micro tubers, and the diameter of the micro tuber.