Biological Activities of Crude Extracts of Some Plant Leaves Cultivated in Vinh Long, Vietnam

Abstract

Abstract This study carried on assessing the in vitro bioactivities of leaf extracts from five plants collected in Vinh Long Province, Vietnam, including extraction yield, antioxidant, antibacterial, and anticancer activities. Five crude leaf extracts were obtained by maceration with ethanol. The antioxidant, antibacterial, and anticancer activities of five extracts were evaluated separately. The results showed that the leaf extraction yields of Vitex negundo, Acrostichum aureum L., Vitex rotundifolia, Bouea macrophylla Griff, and the highest Jasminum subtriplinerve, respectively, were 1.32%, 1.4%, 1.54%, 1.72%, and 2.95%. The leaf extract of B. macrophylla Griff (BLE) had the highest antioxidant capacity, with an IC50 of 6.4 ± 0.08 μg/ml. Except for A. aureum L. and J. subtriplinerve, the other leaf extracts exhibited antibacterial properties against two Gram-positive bacteria and two Gram-negative bacteria. Notably, BLE demonstrated the maximum antibacterial activity against Listeria monocytogenes and Pseudomonas aeruginosa, with inhibition zones ranging from 15 to 16 mm and a MIC of 12.5 µg/ml. Accordingly, BLE was chosen to test for anticancer potential because its activities seemed to be greater than those of the other extracts. Especially, BLE suppressed the proliferation of HepG2 liver cancer cells with an IC50 of 72.33 ± 0.68 μg/ml. In addition, the phenotype of BLE-stimulated HepG2 cell death was typical of apoptotic cell death by analyzing the morphology and DNA fragmentation assay. Overall, the results indicated that five leaf extracts, particularly BLE, were efficient against all tested bacteria and effectively depressed the viability of HepG2 cancer cells. Furthermore, this study’s findings provide foundations for future research into the mechanisms behind these antibacterial and anticancer capabilities, as well as the knowledge of specific treatment pathways.