Proteomic analysis of the effects of low salinity stress on liver of Naked carp (Gymnocyprinus przewalskii)

Abstract

Abstract Naked carp is only distributed widely in Lake Qinghai. A unique feature of this species is its resistance to high salinity, which enables it to survive and adapt to the extreme environment of Lake Qinghai. Since Naked carp is an endangered species, we considered increasing its resources by reproduction and domestication measures in low salinity ponds. To investigate the molecular biological mechanism of the effects of low salinity stress on the liver of the cultured Naked carp, TMT markers combined with high-performance liquid chromatography-mass spectrometry (TMT-LC-MS/MS) were used to compare and analyze the difference in the expression of liver tissue protein between the cultured population (JH) and wild population (QH) in this study. Proteomic analysis results showed that 107 differential proteins were significantly expressed, and 102 of them were up-regulated. Functional and pathway enrichment analysis results found those differential proteins participate in molecular functions such as pyruvate kinase activity, alkali metal ion binding and potassium ion binding, and various biological processes of energy metabolism including glucose metabolism and lipid metabolism. Differential proteins were mainly concentrated in glycolysis and gluconeogenesis, retinol metabolism, linoleic acid metabolism, and steroid biosynthesis and lipid metabolism pathways.