Optimization conditions of adventitious buds regeneration from petiole explants of Echinacea purpurea

Abstract

Abstract Echinacea purpurea as a perennial herb has high ornamental and medicinal value. While the tissue culture technologies of E. purpurea are contradictory. In order to improve the culture conditions of the adventitious buds regeneration of E. purpurea, petioles from the aseptic seedlings were used as the explants. Different concentrations of sodium nitrophenolate (SN), copper sulfate (CuSO4), amine fresh ester (DA-6), sodium nitroprusside (SNP) and ascorbic acid (Vc) were added into MS medium containing cytokinin and auxin, respectively, to investigate their effects on inducing regeneration of adventitious buds. The results showed that the best induction effects of adventitious buds were obtained under 1 mg/L CuSO4, 1 mg/L DA-6, 0.2 mg/L SN, 2 mg/L SNP and 20 mg/L Vc conditions. Our results can provide references for the induction of adventitious buds regeneration and rapid propagation of E. purpurea.