Zebrafish Embryos Immune System induction with BSA as an initial Screening model for Covid-19 Treatment

Abstract

Abstract The early phase of embryonic development involves a lot of signaling and growth factor proteins that will influence the interactions among cells. Administration of BSA (Bovine Serum Albumin) at the beginning of embryo development can interferes organogenesis and causing abnormalities in embryo development. Administrations of BSA were expected to induce responses of the immune system, which be hoped mimicking the stress on immune system caused by infection such as of covid-19. This study aimed to screen the right BSA dose to influence the zebrafish immune system as model animal further study for covid-19 treatment. This research was conducted in the Laboratory of Animal Histology and Embryology, Faculty of Biology, Universitas Gadjah Mada. Eggs were immersed in 1%, 2.5% and 5% of BSA for imemersed in 1 hours and 5 hours repeated 3 times. The Parameter of the study were embryo survivalability rate, morphology, and physiological performance of contraction muscle per minutes and also heart rate per minutes. The data obtained were tested by ANOVA and DMRT test with a confidence level of 95%. The result showed BSA (Bovine Serum Albumin) 1% and 5% has significant effect on normal development and organ function of zebrafish embryos, such as muscle contraction and heart rate of the embryos, as conclusion 1% BSA dose is the most effective responses to induce immune system as model animal further study for covid-19 treatment.