The Percentage of embryo viability after 48h sperm cryopreservation: effect of various natural cryoprotectant

Abstract

Abstract Natural cryoprotectant is an important factor needed to protect cell compound. Its proper usage is based on toxicity which is influenced by its type, concentration, temperature, and exposure period of the compound. This study aims to evaluate the best concentration period of natural cryoprotectant, with the utilized sperm obtained from Tor soro fish using the stripping technique. It was further diluted with a solution consisting of fish ringer, methanol 10%, and natural cryoprotectant in a ratio of 1:10. The natural cryoprotectant used were honey, brown sugar, and date palm juice, at 10 % concentration. Furthermore, the sperm was stored in the liquid nitrogen at -196 °C for 48 h, melted at 36 °C for 1 minute, and mixed with 100 eggs for fertilization. The percentage of embryo viability was analyzed using ANOVA and Tukey test. The result showed an effect of natural cryoprotectant on frozen sperm towards the percentage of embryo viability (p<0.05). The honey cryoprotectant is a natural compound that produces the highest embryo viability percentage at 1080 minutes after fertilization with a total of 94.20 ± 2.79 (p<0.05).