The effect of kersen (Muntingia calabura L) leaf extract on bacteria Aeromonas salmonicida smithia in vitro

Abstract

Abstract Kersen (M. calabura L) leaf can be used as a medicine because kersen leaf contains compounds flavonoids, saponins, polyphenols, and tannins. This study aims to determine the inhibitory power and the smallest concentration of a solution of kersen (M. calabura L) leaf extract to A. salmonicida in vitro. This research uses experimental methods and studied is the activity of kersen leaf extract as an antibacterial to A. salmonicida with a comparison between treatment and control. The treatments were concentrations of kersen leaf extract of 2 mg/ml, 1 mg/ml, 0.5 mg/ml, 0:25 mgml, and 0.125 mg/ml, positive control (chloramphenicol) and negative control, dimethyl sufoxide (DMSO 5% ). The parameters were observed in this study the antibacterial activity of kersen leaf extract to A. salmonicida, and dilution of the concentration of the smallest kersen leaf extract can inhibit A. salmonicida in vitro. The results showed that kersen leaf extract had antibacterial power to A. salmonicida growth. Cassava leaf extract has the ability to inhibit the in vitro growth of A. salmonicida at the smallest concentration of 0.5 mg/ml with inhibiting zone of 10 mm Because at that concentration is more effective when compared with chloramphenicol.