Effect citronella oil against bacteria strains: Escherichia coli ATCC 10536, Staphylococcus aureus ATCC 6538 and Salmonella typhimurium ATCC 14028

Abstract

Abstract A bactericidal property of Cymbopogon nardus oil (CNO) has been investigated in the previous study. This study aimed to confirm the bactericidal activity of Cymbopogon nardus against different bacteria strains. CNO with the concentration of 1%, 3% and 8% were injected into 100 mL canned tubes. Each sample was diluted by hard water and added with a bacterial suspension test in the presence of interfering substance. The mixture was maintained at 20 °C for 5 min. Water shall be new distilled water-not demineralized water. At the end of contact time, an aliquot was taken, and bactericidal activity was immediately neutralized by the diluted-neutralization method. Neutralizers used in this study were: peptone 10 gL−1, beef extract 5 gL−1, NaCl 5 gL−1, soy lecithin 1 gL−1 and polysorbate 80 20 gL−1; while the interfering substance was 0.3 gL−1 bovine albumin for clean conditions. The number of cells in the bacterial suspension test per treatment was 1.54 x 108 cfu mL−1. It was verified that there was no toxic active chemical compounds found after the application of neutralizer and dilution-neutralization method. The output of this study was a bactericidal against strains: Escherichia coli (ATCC®10536™), Staphylococcus aureus subsp. Aureus (ATCC®6538™) and Salmonella enterica subsp. enterica serovar Typhimurium (ATCC®14028™).