Anti-inflammatory effect and chemical constituents of Microcos paniculata total flavone glycosides fraction

Abstract

Abstract Microcos paniculata is an edible and medicinal plant in Lingnan, China. The present study investigated the anti-inflammatory effect and chemical constituents of its’ total flavone glycosides (MpTFG) fraction. MpTFG (10, 15, 20 μg/mL) was applied to 1 μg/mL LPS-induced RAW 264.7 macrophages in vitro; and MpTFG (10, 20, 40 mg/kg body weight) was employed to xylene-induced ear edema test. As a result, 20 μg/mL MpTFG decreased the levels of pro-inflammatory cytokines IL-6, IL-1β and TNF-α in LPS-stimulated RAW 264.7 cells supernatant significantly, and 40 mg/kg MpTFG could effectively ameliorate xylene-induced mice ear swelling. The results showed that MpTFG had potential anti-inflammatory effect. Meanwhile, ten compounds were purified by making use of macro-porous resin, sephadex LH-20 gel column chromatography and semi-preparation HPLC successively. They were characterized to be vicenin-2 (i), isoschaftoside (ii), schaftoside (iii), vitexin (iv), vicenin-1 (v), isovitexin (vi), isoviolanthin (vii), nicotiflorin (viii), astragalin (ix), narcissoside (x) by 1H-NMR, 13C-NMR and HRMS spectral analysis.