Regeneration of Red Palestine FIG (Ficus carica L.) from Formation of Adventitious Shoots in Murashige & Skoog Media with Additional of BAP/TDZ and NAA

Abstract

Abstract The research is aim to determine the regeneration of Red Palestine fig plant by direct or indirect organogenesis; to get the best combination of auxin & cytokinin concentration to induce callus formation; and the best combination of BAP/TDZ and NAA concentrations to regenerate fig plant from adventitious shoot formation. Experimental Treatment I: TDZ 5 mg.l-1; TDZ 7 mg.l-1; 2.4D 4 Kin. 0 mg.l-1; 2.4D 4 Kin. 0.25 mg.l-1; 2.4D 4 Kin. 0.5 mg.l-1; IBA 2 TDZ 1 mg.l-1; IBA 2 TDZ 2 mg.l-1; IBA 4 TDZ 1 mg.l-1; and IBA 4 TDZ 2 mg.l-1. Experimental Treatment II : BAP 2 NAA 0.25 mg.l-1; BAP 2 NAA 0.5 mg.l-1; BAP 3 NAA 0.25 mg.l-1; BAP 3 NAA 0.5 mg.l-1; TDZ 5 mg.l-1 NAA 0.25 mg.l-1; TDZ 5 NAA 0.5 mg.l-1; TDZ 7 NAA 0.25 mg.l-1; and TDZ 7 mg.l-1 NAA 0.5 mg.l-1. The research used randomized block design with 4 replications. The data were analyzed for variance and then Tukey’s HSD at 5% level. The results showed about Red Palestine fig regeneration through indirect organogenesis; the best combination for callus induction were IBA 2 TDZ 1 mg.l-1; and the best treatment for regeneration of adventitious shoots were BAP 2 NAA 0.25 mg.l-1 followed by TDZ 7 NAA 0.25 mg.l-1.