Fungistatic Effect of Ipomea Carnea Extract and Trichoderma Esperellum Against Various Fungal Biological Agents

Abstract

Abstract The study aimed to measure the fungistatic effect of Ipomea carnea and Trichoderma esperellum Tc-Jjr-02 biomass extracts against several fungal isolates of biological agents in vitro. Nine selected biological agent fungi were grown in PDA-chloramphenicol media containing 1% fresh extract of I. carnea biomass, four of them were grown in dual culture with isolate Tc-Jjr-02 with an incubation period of 96 hours. The results showed that the I. carnea biomass extract had a fungistatic effect by inhibiting the growth of the entomopathogenic agents Metarrhizium anipsoliae Me-Sdj-16 (27.8±3.2%) and Beuveria bassiana Be-Sj-13 (38.1±2.6%) and Be-Sdj-15 (18.6±1.0%), and the biofertilizer agents Aspergillus sp. As-Sdj-11 (46.7±1.9%), Pénicillium sp. Pc-Sdj-14 42.7±3.5%, and Trichoderma sp. Tc-Sdj-18 38.5±1.0% at 96 hours incubation period (HAI). The biocontrol agent T. esperellum gave a weak fungistatic effect against the biofertilizer agents As-Sdj-11 and Pc-Sdj-07 were 14.4±1.3% and 7.0±1.9% at 96 HAI and did not have a fungistatic effect on the biofertilizer agents Trichoderma Tc-Ba-05 and Tc-Sdj-09. Application of I. carnea biomass as green manure and some biofertilizer agents can be carried out simultaneously; while the application of T. esperellum can be carried out using biofertilizer agents As-Sdj-11 and Pc-Sdj-07 and Tc-Ba-05 and Tc-Sdj-09.