The Effect of Humic Acid on some Chemical Characteristics of the Leaves of Date Palm off Shoots of Nabut Seif Cultivar Resulting from Tissue Culture Under Salt Stress Conditions

Abstract

Abstract College of Agriculture and Marshes (COAM), Thi Qar University, conducted this study for the season 2018-219 to explore the effect of sodium chloride, humic acid and their interrelationship on some chemical characteristics of leaves of date palm offshoots of the Nabut Saif cultivar obtained from tissue culture. The study involved (12 treatments), each with three replicates. Considering that they were derived from 36 offshoots of the Nabut Seif cultivar, similar in size, length, number of leaves and three years old. The experiment was designed as a factorial experiment according to the complete randomized block design (C.R.B.D) and with two factors: the first factor is the use of four concentrations of sodium chloride salt (0, 5, 10, and 15) dsm-1 while the second factor is three concentrations of humic acid 80% (0, 10 and 20) ml L-1, and both factors were added to the soil in ten separate applications in between every 15 days, besides, the study showed the following results: The sodium chloride had a considerable impact on the elevation of the concentration of total soluble carbohydrate, proline and the activity of superoxide dismutase enzyme in the leaves in response to its increasing level added to the soil, with the concentration of 10 dsm-1 recorded the highest average for each carbohydrates (25.24 µg g-1), proline (5.27 µg With respect to the impact of humic acid, this was substantial since it increased the levels of total soluble carbohydrates, proline, and the activity of the superoxide dismutase enzyme with an increase in the applied concentration, the concentration treatment (20 ml L-1) achieved the highest averages of carbohydrates (25.26 µg g-1), proline (5.60 µg g-1). The interaction treatments of the study factors significantly affected all the characteristics studied by increasing their mean values whereby the interaction of sodium chloride at a concentration of (10 dci) and humic acid at a concentration of (20 ml L-1) excelled and achieved the highest means of carbohydrates (26.32 µg g-1), proline (6.16 μg g-1).