Antioxidant Effect of Clerodendrum sp and Acanthus illicifolius Methanol Extraction on Blood Profile of Male Mice Induced by Benzo(α)pyrene

Abstract

Abstract Benzo(α)pyrene is one of carcinogenic PAH substances which commonly produced by many different sources of polutant. The study was conducted in order to determine the effect of methanol extraction of Clerodendrum sp and Acanthus illicifolius which known to be two of common mangrove plants on the blood profile of male mice induced by benzo(α)pyrene (one of polycyclic aromatic hydrocarbon/PAH). Thirty male mice (Mus musculus) of ddy strain from Lampung Vetenary Agency were used to conduct the study. Completely randomized design was assigned for this study. Mice were grouped into 5 different group treated with PAH injection, one groups used as control without any injection, one as postitif group with PAH injection but not given with any methanol extraction and the last three group were given with methanol extraction of Clerodendrum and Acanthus and taurine prior PAH injection. All the animal study were kept in room temperature with 12:12 light cycle and fed and water ad libitum. Observation of their blood cells and protein profile was made after 14 days. Data was analyzed by One-Way Anova at 5% followed by Fisher’s at level of p < 0.05. The study showed that methanol extraction of Clerodendrum, Acanthus and taurine were able to return back either the number of redblood or whiteblood cells. However, the protein profile of the blood was not able to show any differences among treatment groups from which 14 different bands were observed under SDS-PAGE method.