Author:
Hayati R,Basyuni M,Purba A,Arifianto D,Syahputra I,Chalil D,Bimantara Y
Abstract
Abstract
Method of CTAB (cetyltrimethylethylammonium bromide) is prevalent for low-cost DNA extraction with many published variations for other plants and tissues, including fungi Ganoderma. The quantity of DNA extracts from the Ganoderma sp fungus from a coconut tree and rubber tree with concentration extraction efficiency and the quality of purity wholeness varied with different DNA characterizations. DNA purity was measured using UV spectroscopy, where a ratio of 260 nm, 280 nm 320 nm was an indicator of different contaminants. The quantity of DNA from the isolate is indicated by 31 ng/ul until 133 ng/ul and range of ratio from 1,279 until 3,6654. The DNA extraction quantity ratio value obtained is below, and above the average, for absorbance 260 nm, 280 nm and 320 nm varies between 1.5 until 1.8 which is an indicator of DNA purity. Pure DNA isolate without contaminants is only found in sample isolate Ganoderma sp 141.1 from coconut tree with a ratio value of 2.2707. Furthermore on the other samples thought to be still contaminated with phenols, salts, proteins, polysaccharide or RNA still co-extracted with phenolic acid (chloroform phenol).
Reference15 articles.
1. Role of benzoic and salicylic acids in the immunization of oil palm seedlings-challenged by Ganoderma boninense;Surendran;Ind Crops Prod.,2018
2. Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR;Karthikeyan;Plant Protect. Sci,2006
3. Laboratory investigations on fungicides and biological agents to control three diseases of rubber and oil palm and their potential applications;Jollands;Pest Manag Sci.,2008
4. In-vitro Antagonism Experiment of Trichoderma Spp. to Ganoderma SP. Which Attacks Sengon Trees;Dendang;J FOREST RES-JPN,2015
5. RAPD markers on genetic diversity in three populations of pisifera type of oil palm;Basyuni;(Elaeis guineensis) IOP Conf. Ser.: Earth Environ. Sci.,2018