Virulence of Spodoptera Litura Nuclear Polyhedrosis Virus (SLNPV) with kaolin as carrier material on spodoptera litura and tetragonula laeviceps on soybean

Abstract

Abstract The objective of research is determined the virulence of different concentrations of SlNPV-JTM 97C with kaolin as carrier material on S. litura and T. laeviceps mortality on soybean. The Research was conducted in Plant Protection Laboratory of State polytechnic of Jember, from June to September 2020. The lethal concentration (LC50 and LC95) SlNPVusing Polo Plus 1.0, with the IRAC version 3, method number 008. The S. litura mortality test used Completely Randomind Design with 5 treatments and 3 replications. The treatments were contamination of larvae diet by SlNPV with control (the third instar larvae without contamination SlNPV), 0,1%, 0,2%, 0,3% and 0,5%. These concentrations are equivalent to the concentrations of 0.1.2x 108 PIBs/g, 2.4x 108 PIBs/g, 3.6 x 108 PIBs/g and 6.0 x 108 PIBs/g, respectively. Each test was repeated three times. Mortality percentage of larvae were observed as experiment data. Those data were analyzed by Analysis of Variant and Least Significant Difference (LSD) 5%. SlNPV virulence test against T. laeviceps using a concentration that can cause S. litura mortality ≥ 70%. The results of the research indicated that LC50 of SlNPV with kaolin as carrier against S. litura 3rd instar was 0.2%, LC95 was 0.5%, effective concentration of SlNPV to control S. litura 3rd instar was 0.3% to 0.5%, SlNPV concentration at 0.3% to 0.5%, non-toxic to T. laeviceps.