Changes in the taxonomic composition of the rumen microbiome during the dietary supplements administration

Abstract

Abstract The degree of the dry matter (DM) digestibility of the diet and the effect of additional administration of linseed oil on the composition of the rumen microbiome of cattle were studied. The in vitro method was used using the incubator "ANKOM Daisy II "(modifications D200 and D200I) according to a specialized technique. Microflora analysis was performed using MiSeq ("Illumina", USA) by the new generation sequencing method (NGS) with a set of MiSeq reagents. With the introduction of linseed oil, an increase in the digestibility of DM feed by 2.3% relative to the control (wheat bran) was noted. In the experimental group the level of species diversity was 45% higher than in the control group. The Simson dominance index in the control group was 0.44, in the experimental group it is 0.54. The Shannon index was equal to E=0.3, which shows a greater alignment of the microbial community in the ruminal fluid when flaxseed oil was included. There was an increase in the number of bacteria of the phylum Bacteroidetes (64.2 %) and a decrease in Firmicutes (19.3 %)., There was an increase in the number of microorganisms belonging to the classes Saccharibacteria, Spirochaetia, Mollicutes, Lentisphaeria, Elusimicrobia in the experimental group, relative to the control. Also it is an increase in representatives of all classes of the phylum Proteobacteria in comparison with the control by 44 % (p < 0.05).