Detection of pathogenic bacteria in the surgical rooms of Al-Ramady hospital using next generation sequencing

Abstract

Abstract This study was aimed at applying the next generation sequence technique, to evaluate the microbial contamination of the General Al-Ramady Hospital and the diagnosis of dangerous types. Several surface swabs were taken from the surgical room devices and equipment used during the surgery period at the hospital. Hospital environment contributes chiefly to the emergence of Nosocomial infection, the most common and severe complications in the provision of health care to patients in the hospital, it has become necessary to specify and identify the causes of illness through alternative methods to traditional methods that waste time, money and effort.. The results showed contamination of hospital environment by several varieties of bacteria. They were pathogenic and nonpathogenic bacteria, in spite of the action of the Antiseptics used in sterilization. the most common of which were several types of Pseudomonas of 84.26% and Acinetobacter of 13.85%. The present of this pathogenic bacteria in hospital-related injuries, which are mean vital indicators of contamination in the hospital environment. Data suggest that it is possible to diagnose and identify the types of pathogenic, discover effective strategies for monitoring and controlling the hospital environment and providing the essential tools to avoid the spread of infection, particularly in the presence of the molecular technique.