Chitinase production by Trichoderma viride in submerged state fermentation

Abstract

Abstract We have identified the optimal conditions for the production of chitinolytic enzymes of T.viride in submerged state fermentation. The production of chitinase by a new strain of fungus was carried out on the basal liquid medium, containing (%) colloidal chitin 0.5, NaNO3 0.2, KH2PO4 0.1; MgSO44-7H2O 0.05 and KCl, 0.05. The activity of enzymes of the chitinase complex of the strain was evaluated using the method using dinitrosalicylic acid (DNS reagent). A quantitative determination of the activity of chitinases in a producer microorganism was established by their ability to hydrolyze 0.2% colloidal chitin (in phosphate buffer 0.05 M, pH 5.2), by the content of reducing sugars formed in this process, which were evaluated using a DNS reagent. The results of studies of the influence of various cultivation parameters showed that highest chitinolotic enzymes production by T.viride was obtained at pH 4.0, (301.15-303.15) K and after 144 h growth. The studied soil isolate can be further used in biotechnological research, as well as for biological control of pests and pathogens of agricultural crops.