Preliminary Study on Antimicrobial Activity of Fermentation Broths of Oudemansiella Mucida

Abstract

Abstract Oscillation culture was carried out on Oudemansiella mucida., the obtained fermentation broths were extracted with ethyl acetate, n-BuOH in succession to give three sections: extract of ethyl acetate, n-BuOH and the remained fermentation broths. Their antimicrobial activities against five common phytopathologenic funguses were determined by filter paper method and solid culture medium method. The result indicated that the fermentation broths markedly inhibited five phytopathologenic funguses. The metabolites with antimicrobial activity were mainly congregated in EtOAC extract, with the minimal inhibiting concentration (MIC) for Alternaria brassicae, Alternaria longipes and Gloesporum fructigenum being 10 mg/mL, the MIC for Fusarium graminearum and Alternaria alternata being 5mg/mL.