Radiosensitivity of post-gamma irradiated Indigofera zollingeriana

Abstract

Abstract Gamma Irradiation by employing gamma-ray enables genetic transformation to produce the target gene activation functioning as a productivity determinant. A number of studies had found that the employment of gamma-ray is capable in improving the plant’s tolerance against drought treatment of irradiation. The purpose of this study was to determine the Lethal Dose 50 (LD50) and Reduction Dose 50 (RD50) of Indigofera zollingeriana post gamma irradiated. The irradiation dosage consisted of 0Gy (control, 50 Gy, 100 Gy, 150 Gy, and 200 Gy. Each dose consisted of 250 seeds were sown in the tray for 2 weeks after germination to determine the values of LD50 and RD50. Each LD50 and RD50 value of the Indigofera seeds was determined by processing the data on the survival rate of the radiation dose treatment survival rate using the Curve Expert 1.3 program. The observed parameter encompassed the germination percentage, seedling length, and seedling vigor index. This research shows the percentage of germination showed a value of 45.125 + 0.11 X -0.0006 x2 with LD50 = 320. 282 Gy; seedling length = 4.156 - 0.00472 X, with RD50= 406.356 Gy; and Seedling vigor index = 153.916 - 0.31244 X with RD50 = 246.435 Gy.