Measurement of quality non-specific and specific-parameters of 70% ethanol extract and simplicia from cascara coffee robusta (Coffea canephora L.) and its potency as antioxidant.

Abstract

Abstract Cascara coffee robusta (Coffea canephora L.) has been previously studied and has been reported to have biological activity with potential antibacterial and antioxidant effects. The ethanol extracts of cascara coffee robusta contained secondary metabolites: flavonoids, alkaloids, steroids, terpenoids and tannins. Measuring the quality of natural ingredients is an important means of maintaining the quality and safety of natural medicines. The purpose of the research was to measure the quality of non-specific and specific-parameters of extract and 70% ethanol and simplicia from cascara robusta coffee and evaluate its antioxidant activity. Material and Methods: Samples were soaking with ethanol 70% and evaluated for antioxidant activity using the DPPH method. Results: The results showed that the specific parameters have the same organoleptic properties as simplicia and a 70% ethanol extract of cascara coffee robusta are dark brown, characteristic odor, slightly bitter taste. Water soluble and ethanol soluble 70% ethanol extract of cascara has a total content of 51.98% ± 0.31 and 68.36% ± 1.74. Phytochemical screening results for 70% ethanol extract and simplicial of cascara coffee robusta include alkaloids, flavonoids, saponins, terpenoids, steroids, and tannins. Non-specific parameters of cascara coffee robusta simplicia; total water content of 3.26% ± 0.44; Total ash 4.91% ± 0.16; acid-insoluble ash 0.55% ± 0.33; <1.0 x 100 CFU / gr fungal and yeast contamination; <1.0 x 100 CFU / gr microbial contamination. Non-specific parameters of cascara coffee robusta extract; total water content of 3.55% ± 0.57; total ash of 6.15% ± 0.00; total acid-insoluble ash 0.23% ± 0.02; <1.0 x 10^0 CFU / gr fungal and yeast contamination; <1.0 x 10^0 CFU / gr microbial contamination. Ability the antioxidant activity was demonstrated by the IC50 values of simplicia and the extract, ie 55.14 ± 1.90 μg/mL simplicia and 57.40±0.29 μg/mL extract. Conclusions: Statistical tests show that there is no difference between the values of specific, non-specific parameters and their antioxidant capacity of simplicia and the extract, and all parameters follow standard procedures as a reference requirements. Showed that it meets.