Culture of potato microspores (Solanum tuberosum L.) Nevsky variety

Abstract

Abstract Within 24 days of cultivation, the ratio of the number of potato microspores at different stages of development was studied depending on the content of 6-BA in nutrient media. Four media options were used: No. 1 MS + 6-BA 1 μM, No. 2 MS + 6-BA 2 μM, No. 3 MS + 6-BA 3 μM, No. 4 MS + 6-BA 4 μM. It was found that the use of MS-based culture media with 1-4 μM 6-BA is effective for the induction of embryoidogenesis in potatoes in microspore culture. 88.0-95.0% of the microspores were in a living state for 24 days. The frequency of embryoid formation in the experiment was 0.5-1.0% of the total number of microspores and 4.7-6.6% of the number of multicellular structures. Monad could be the initial cells of the embryoids of the tetraploid variety of potato in the period up to 11-12 days. In addition to single germ-like structures, in the suspension of potato microspores, embryoid conglomerates - polyembrioids - were noted on days 11-12. In the light, microspores and germ-like structures of Nevsky tetraploid potato variety acquired a green color. On the 24th day of cultivation, developing potato embryoids were detected in the phase of organogenesis.