Study on HPLC Fingerprint of Clematis manshurica Rupr from Different Areas-Reference-Cited by-同舟云学术

Study on HPLC Fingerprint of Clematis manshurica Rupr from Different Areas

Published:2019-05-01 Issue:6 Volume:267 Page:062053
ISSN:1755-1307
Container-title:IOP Conference Series: Earth and Environmental Science
language:
Short-container-title:IOP Conf. Ser.: Earth Environ. Sci.

Author:

Kong Juan,Huang Xulong,Yang Xiaosong,Zhang Lingling,Li Xiaofen,Zeng Qian,Wu Hongmei,Wang Xiangpei

Abstract

Abstract Objective: To establish HPLC fingerprint of Clematis manshurica Rupr from different areas. Method: 10 batches of Clematis manshurica Rupr was analyzed by RP-HPLC that the methods were used Odyssil-C18 chromatographic column, with the mobile phase of acetonitril-0.1% phosphoric acid solution, the flow rate was 0.8mL/min, the wavelength was 210 nm, and column temperature was 30°C. And the similarity was evaluated by similarity evaluation software. Result: The HPLC-UV fingerprint of Clematis manshurica Rupr was established, and 20 common peaks were determined, the similarity was between 0.974-0.823, indicating that the differences of Clematis manshurica Rupr from different regions were significant. Conclusion: The analytical method has good separation degree and feasibility, and it can provide a reliable basis for the quality control of Clematis manshurica Rupr.

Publisher

IOP Publishing

Subject

General Engineering

Link

https://iopscience.iop.org/article/10.1088/1755-1315/267/6/062053/pdf

Reference7 articles.

1. Progress in the study of components, pharmacological activity and clinical application of Clematis chinensis [J];Zhao;Chinese medicinal materials,2008

2. Research status of Clematis manshurica Rupr [J];Zhang;Heilongjiang Medicine,2011

3. Isolation and identification of chemical constituents from Clematis manshurica Rupr [J];Liu;Chinese Journal of Pharmaceutical Chemistry,2016

4. Advances in studies on chemical constituents and pharmacological activities of Clematis chinensis [J];Luo;Journal of Gansu College of traditional Chinese Medicine,2015