Utilization of Double Fungal Treatment by Trichoderma harzianum and Saccharomycopsis fibuligera to produce biosugar from red seaweed Kappaphycus alvarezii

Abstract

Abstract Kappaphycus alvarezii is one of the natural resources that contains high carbohydrates and has the potential as an alternative raw material for the sugar industry. The aims of this research is to analyze the optimal conditions of the combination of Trichoderma harzianum and Saccharomycopsis fibuligera in degrading polysaccharides from K. alvarezii into sugar. T. harzianum and S. fibuligera were activated three times at 30° C in 2% seaweed substrate. The method used was a combination of hydrolysis and fermentation to produce evaluated bio sugar in various concentrations of inoculum (5%, 10%, 15%, and 20%) and time (0 hour, 24 hours, 48 hours, and 72 hours). Based on the results, hydrolysis with an inoculum concentration of 20% for 24 hours showed the highest total sugar production. Followed by fermentation with S. fibuligera which produced glucose and galactose which is 1.74 g/L and 9.93 g/L. In conclusion, this research demonstrates the ability of T.harzianum and S. fibuligera to convert carbohydrates from K. alvarezii to produce biosugar through a combination of hydrolysis and fermentation methods.