Abstract
Abstract
As a result of molecular genetic monitoring of bovine leukemia based on a set of methods (gene diagnostics - polymerase chain reaction (PCR), automatic sequencing; molecular phylogenetics) phylogenetic analysis presents the structure of an improved own replenished database (DB) for the characterized nucleotide sequences of the bovine leukemia virus (BLV), which includes information arrays contained in interconnected tables, which allows accumulating, storing, and combining all data through the formation of structured queries and reports with specified parameters in the database management system. At the same time, the results of the study are presented on the prevalence of BLV in the territories of some regions of Russia and Ukraine among the livestock; comparative analysis of genetic variants of the virus by genome loci: pol with clustering of the studied isolates into clade 1; env with the identification of 5 genotypes (GI (4%), GII (6%), GIV (79%), GVII (10%), GVIII (1%)) with the dominance of genotype IV (“European cluster”). In the course of the study, a low level of genetic variability among field isolates of BLV was established for the pol - 1.9% and env - 0.2-1.8% gene loci (depending on the genotype). This information product is registered in the register of databases: certificate of state registration No 2020620530 dated 23.03.2020.