Universal biotechnological medium for sperm dilution during poultry artificial insemination

Abstract

Abstract The aim of the research was to develop a universal biotechnological environment for diluting sperm during artificial insemination of birds. The creation of the medium was carried out in 4 stages. The first stage is the selection of environmental components of their influence on the survival of sperm cells in vitro. During the second one, the combination of components and their effect on sperm of different species of birds was studied. Subsequently, the effect of the complex of combinations of ingredients on the preservation of sperm motility was determined upon dilution of the ejaculates of roosters, turkeys, cesars, drakes, husaks during in vitro storage. Ejaculates of males of the studied species in quality close to the average species value were used. During the experiments, the ratio of chemical components was determined, which ensured the optimal value of the concentration of hydrogen ions when the medium was diluted in distilled water. The permissible pH of the medium has been established; it must be neutral or slightly alkaline. The pH was adjusted by injecting sodium acetate. The created medium is stored in a dry state for up to 2 months at a temperature of + 6–8 ° C in hermetically sealed containers. Sperm diluted with this medium contributes to maintaining the high fertilizing ability of rooster sperm for up to 24 hours, for turkeys and cesaries for 7 hours, for waterfowl for 3-4 hours.