Pigments constituents, phenolic content and antioxidant activity of brown seaweed Sargassum sp

Abstract

Abstract Seaweed contains many biofunctional compounds such as pigment and antioxidants that have the utility to inhibit degenerative diseases that are caused by reactive species oxygen (ROS). This research aimed to measure the constituents of photosynthetic pigment and antioxidant activity of brown seaweed Sargassum sp. from North Sulawesi. Sargassum sp. was extracted using n-hexane, acetone, and ethanol by the maceration method. The research consisted of chlorophyll (a, b, and C1+C2), fucoxanthin, carotenoid, phycocyanin and phycoerythrin, total phenol content (TPC), scavenging radical activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP). The result exhibited that the hexane extract contained higher chlorophyll a and fucoxanthin than ethanol extract. The TPC values of acetone and ethanol extract of sargassum sp extract were 45.382 ± 3.023 and 44.885 ± 2.834 μg GAE (Galic Acid Equivalent) g-1 extract, respectively. Acetone extract of Sargassum sp. had a lower antioxidant activity of DPPH radical scavenger (IC50 2.966±0.178 mg mL-1) than ethanol extract IC50 2.684±0.256 mg mL-1 extract). The FRAP value of acetone and ethanolic extract of S.olygocystum were respectively 14.45±1.425 and 9.09±0.876 μM Fe2+ mg-1. The Sargassum extract is a potential natural source of natural pigment and antioxidant.