A Recyclable Polymer-supported Glycane as pH Buffers for Urease-catalyzed Reaction

Abstract

Abstract we utilized the PSG beads as polymer-supported buffer agents. Ammonia was measured by the Berthelot method, Buffer activity of the PSG buffers in enzyme-catalyzed reactions at neutral solition was examined for its ability to control the pH of urease catalysed hydrolysis of urea. The urease activity of soybean which analysed using PSG buffers in pH 7. The recycling ability of the PSG was tested. In each cycle, the PSGs were regenerated by HCl and changed into mixed H-Na form by NaOH (H+ form 40% and Na+ form 60%). We demonstrated that PSG exchangers show high buffer activity at neutral pH. The PSG exchangers were effectively used to control the pH of urease catalyzed reactions. The new buffers are as efficient as phosphate buffers in neutral solution but more environmentally benign.