Target gene prediction and pathway analysis of miRNA targeted AMPK involved in lipid accumulation of bovine granulosa cell luteinization

Abstract

Abstract Luteinization is process of turning granulosa into luteal cells to produce progesterone. It’s indicated by lipid accumulation which controlled by AMPK gene, the expression is controlled by miRNAs at pre and/or post transcriptional level. Previously, 4 miRNAs namely miR-19b, miR-130, miR-101, and miR-19a were predicted targeted to AMPK. For that, this study aimed to identify board target of these miRNAs to genes in specific metabolic pathway. An insilico study was performed using online molecular databases that are miRDB (www.mirdb.org) and DAVID Bioinformatic Resource (https://david.ncifcrf.gov/) and Genomes (KEGG) pathway (https://www.genome.jp/kegg/pathway.html). Result shows that miR-19b, miR-130, miR-101, and miR-19a targeted to 1121, 738, 859, and 1117 respectively. A total 3835 genes were analyzed, resulted five annotated functional group namely coiled coil, serine/threonine-protein kinase, ATP-binding, nucleotide-binding, and kinase. According to gene ontology analysis, genes were grouped at nucleoplasm, cytoplasm, nucleus, golgi apparatus, and transcription factor complex process. Moreover, pathway analysis found five top KEGG pathways that are FoXO, MAPK, cAMP, Sphingolipid, and prolactin signaling. Two genes namely RAF1 and AKT3 were involved in all pathways. It is concluded the expression of miRNA group targeted to AMPK affect to complex cellular metabolism pathway direct and/or indirectly.