Comparative study of herbal and non-herbal egg protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Abstract

Abstract Chicken egg yolk and albumen contain some bioactive proteins. The present study was conducted to compare the protein profile of yolk and albumen of herbal and non-herbal chicken egg. The herbals that supplemented into chicken feed consisted of turmeric, sambiloto leaves, soursop leaves, ginger and lemongrass. The protein profiles were measured in term of the numbers of bioactive peptide fraction and their molecular weight by SDS PAGE using descriptive qualitative analysis. The antioxidant binding protein were also evaluated. The result show that SDS-PAGE can separate the egg yolk protein fraction and albumen protein with molecular weight between 14-97 kDa. Egg yolk protein fraction of herbal eggs separated better than non-herbal eggs, whereas giving herbal to laying hens does not affect the number of fractions in albumen protein. Antioxidants from herbals were indicated binding the apoprotein and apolipoprotein of egg yolk, also avidin and ovalbumin of albumen. It can be concluded that herbals supplementation affects the bioactive protein fraction in chicken eggs.