The effect of 0.6 and 0.8% Bovine Serum Albumin (BSA) levels in the Cauda Epididymal Plasma-2 (CEP-2) diluent to maintain Ongole Crossbred post-thawing motility sperm

Abstract

Abstract Decreasing in post-thawing sperm motility of Ongole Crossbred (OC) influences the success of conception through artificial insemination (AI). Sperm membrane damaged during frozen storage is assumed as the cause of a decrease in post-thawing motility. Sperm motility is one of the critical indicators of frozen semen processing AI purposes. This study was aimed to assess the best BSA levels in Cauda Epididymal Plasma-2 (CEP-2) diluent in maintaining sperm post-thawing motility. This study used fresh ejaculate of a three-year-old bull, collected once a week using artificial vagina. The experiment was based on BSA level 0; 0.6 and 0.8% in CEP-2+10% egg yolk. The results showed that different BSA levels in CEP-2+10% egg yolk diluents were able to maintain post-thawing sperm motility: 42±2.58; 44±2.11, and 44±2.11% for control (0%), 0. 6% and 0. 8% BSA level, respectively. It is concluded that CEP-2+10% egg yolk + 0.6% BSA level effectively maintained sperm post-thawing motility.