Post-thawing sperm quality of Boer buck semen diluted in phosphate buffer saline supplemented with bovine serum albumin

Abstract

Abstract This study aimed to determine post-thawing sperm quality of Boer buck which diluted in Phosphate Buffer Saline (PBS) medium supplemented with Bovine Serum Albumin (BSA) for optimizing its quality in addressing In Vitro Fertilization (IVF) purposes. In this research thawed semen was diluted in PBS with the addition of three different BSA concentration namely T0 (BSA 0%), T1 (BSA 0.1%), and T2 (BSA 1%). Semen and sperm quality such as motility, viability and abnormality (all in %) were observed at initial thawing and after diluted with PBS and BSA before and after incubation. At initial thawing, semen characteristic shows motility at 62.67±7.03%. Meanwhile, in all sperm quality after dilution at T0, T1 and T3 shows decreased after incubation time, although no differences found between BSA concentration (P>0.05). From this study, it is concluded that adding BSA as complementary nutrition in PBS medium could maintain sperm quality at affordable level for further IVF purpose.