Experiment study on the treatment effect of the hydrocarbon degradation microorganism to treat the oily wastewater

Abstract

Abstract In view of the large amount of oily wastewater, the complexity of pollutant composition and the difficulty of effective treatment, the idea to adopt the microbial technology to treat it is put forward and the influencing factors of oil-degrading microbial treatment of oily wastewater.optimal degradation conditions and degradation kinetics have been studied in depth. The degradation effect and the inflluecing factors of the hydrocarbon degradation microorganism to treat the oily wastewater were investigated in this study. Firstly, the hydrocarbon degradation microorganism which can degradate the oil pollutions quickly and effectively are screened and identificated and the physiological and biochemical characteristics are detaily reaearched.. Secondly, the influencing factors and effects of oil-degrading bacteria treatment of oily wastewater and the optimal degradation conditions, which including pH value, rude oil mass concentration and the biological inoculum size are studied in depth and determined. Thirdly, the degradation kinetics characteristics of the petroleum hydrocarbon degrading stains are investigated. At last, the degradation effect of the oil pollution under the optimal condition is studied as the focus research is studied to verify the biodegradation effects of the strains in order to provide some technical reference for the practical application of this technology. The research results indicate that: (1) the Petroleum hydrocarbon degrading bacteria we screened all have good treatment effect on oily wastewater; (2) Analytical identification of morphological and physiological and biochemical characteristics indicated that both JA and JB are all Pseudomonas sp and their optimal growth times are 18-23h, and 20-21h respectively; (3) The degradation effect of the mixed strain. on the pollutants is much better than that of the single one, Under the JA to JB mixed rate of 1:2, the average degradation rate of crude oil can be increased from 23.4% of the single bacteria to 49.6% of the mixed one; (4) The most important influencing factors affecting the degradation performance of petroleum-degrading bacteria are pH value, followed by crude oil mass concentration and biological inoculum. Among them, under the condition of pH 7.15, crude oil mass concentration of 3387mg/L and biological inoculum size of 75ml/L, the mixed bacteria had the highest degradation rate of crude oil, up to 55.76%.