Identification of active compounds from lindur root plants (Bruguiera gymnorrhiza) as α-glucosidase inhibitors

Abstract

Abstract Lindur (Bruguiera gymnorrhiza) is a plant that contains bioactive sources for antioxidants and α-glucosidase inhibitors. The α-glucosidase inhibitors are inhibitors of the α-glucosidase enzyme involved in digestion in the intestine. This study aimed to determine and select the potential inhibitory activity of the α-glucosidase enzyme from the crude extract of lindur by in vitro. This research was carried out through several studies that involved multilevel extraction. testing α-glucosidase inhibitors from crude extracts of lindur root. Fractionated from active extracts of lindur used thin layer chromatography (TLC) & preparative thin layer chromatography (PTLC). and combined compositions bioactive from the most active fraction. The highest yield was obtained from root ethanol extract which was 4.68. Ethanol extract of stem and root bark had an activity of α-glucosidase enzyme inhibitors calculated by IC50 values 171.31 ppm and 153.07 ppm. The fractionation results used preparative (PTLC) showed 5 fractions in crude ethanol root extract. Fraction 1 with Rf 0.15 had the most potential activity of α-glucosidase enzyme inhibitors (161.05 ppm). Identification chemical composition used by Gas Chromatography Spectrometry (GC-MS) showed the composition contained in the fraction 1 of the root ethanol extract mostly (similar to> 90%) was hexadecanoic acid or palmitic acid and phenol.