The administration of Caulerpa racemosa extract on total bacteria and survival rates of white shrimp (Litopenaeus vannamei) after infected by Vibrio parahaemolyticus

Abstract

Abstract The purpose of this study was to determine the effect of administration Caulerpa racemosa extract on total bacteria and survival rate of white shrimp (Litopenaeus vannamei) after infected by Vibrio parahaemolyticus. This study used a Completely Randomized Design with five treatments and three replications. Shrimp were divided into four groups, one control group without C. racemosa extract, another three groups were administrated with C. racemosa extract in different doses; 3 μg/g (P1), 6 μg/g (P2) and 9 μg/g (P3) by injection. After 24 hours, the control group was divided into two groups, negative control (K-) was injected by PBS and positive control (K+) also another three groups (P1, P2 and P3) were infected by V. parahaemolyticus. Observed parameters were total bacteria and survival rates. On the 1st day, the total bacteria was lower in the treatment with C. racemosa extract than K+ and significantly different (P <0.05). On the 7th day, total bacteria increased again, but in P3 (20.46 ×104 CFU/ml) was lower than K+ and significantly different (P <0.05). The highest survival rates was 93.33% (P2) and the lowest was 56.67% (K+). It can be concluded that C. racemosa extract can be given to L.vannamei at dose 6 μg/g.